Introduction
Cryopreservation is essential for long-term storage of biological samples, suspending cellular metabolism at ultra-low temperatures (-80°C to -196°C) to maintain viability. Esco’s Liquid Nitrogen Containers (LBC & LC) offer secure and efficient cryogenic storage for cells, blood, gametes, and other critical samples. Featuring advanced insulation, lightweight yet durable aluminum construction, and optimized designs for easy handling, they minimize LN2 evaporation while ensuring maximum sample protection. Ideal for biobanks, research labs, hospitals, and IVF clinics, Esco’s LN2 storage solutions provide reliability for preserving valuable biological materials.
Must-have features to consider:
- Superior heat insulation technology minimizes liquid nitrogen evaporation.
- Multi-layer insulation with low thermal conductivity ensures a uniform temperature.
- Aluminum foil with high reflectivity reduces heat transfer.
- Five-year vacuum quality guarantee ensures long-term stability and performance.
- Threaded structure provides secure sealing and leak-proof design.
- High-strength aluminum alloy construction offers durability and longevity.
- Available in various sizes to accommodate different storage needs.
Liquid Nitrogen Biological Container Square Canister Series is ideal for laboratories, blood banks, hospitals, disease control centers, and research institutions requiring secure long-term storage of biological samples, cells, cord blood, and gametes.
Liquid Nitrogen Biological Container Round Canister Series is designed for hospital reproductive medicine centers and livestock breeding institutions, ensuring proper preservation of sperm, eggs, embryos, and frozen semen in straws for artificial insemination and embryo transfer services.
LBC-_
Liquid Nitrogen Biological Container Square Canister Series
Specifications:
- Made of lightweight, high-strength aluminum.
- Round canister design for efficient static storage.
- Low LN2 consumption minimizes refill frequency.
- Maintains ultra-low temperatures from -180oC to -196oC.
- Available sizes: 35 L, 47 L, 50 L, 65 L, 95 L, 120 L, 145 L, 175 L.
Cryopreservation is the process of preserving biological samples at extremely low temperatures, typically between -80°C to -196°C, to halt cellular metabolism and maintain long-term viability. This technique is crucial for research, medical treatments, and biobanking
Liquid nitrogen biological containers are designed to store various biological samples, including cells, blood, sperm, eggs, cord blood, and other critical specimens used in medical and research applications.
LBC is specifically designed for long-term storage of cryovials using square racks and cryoboxes, making it ideal for research and clinical use. In contrast, LC is used for storing straw tubes inside round canisters with aluminum canes, commonly used in reproductive and veterinary applications.
Before use, inspect the container for any visible damage. Pre-cool it by filling one-third of its capacity with liquid nitrogen, allowing it to stabilize for about two hours before completely filling it. This helps prevent thermal shock and ensures optimal performance.
The liquid nitrogen level can be measured using a level gauge ruler (1m) designed for precise readings. Avoid using hollow tubes or inappropriate tools, as they can cause splashing and pose safety risks.
It is recommended to monitor the liquid nitrogen level regularly and refill when it drops below 30% of the total capacity. This ensures that samples remain in optimal cryogenic conditions and are not exposed to temperature fluctuations.
Since liquid nitrogen evaporates into nitrogen gas, it can displace oxygen in enclosed spaces, creating a risk of asphyxiation. Proper ventilation is essential, and in confined areas, an oxygen detector should be used to ensure safety.
Always wear cryogenic gloves and protective eyewear when handling liquid nitrogen to prevent frostbite or burns. Avoid touching metal surfaces directly, as they can cause skin damage. Additionally, never tamper with the vacuum port and always keep the neck plug dry to maintain insulation efficiency.
If excessive condensation or ice buildup appears on the container's exterior, it may indicate a vacuum loss, leading to increased liquid nitrogen evaporation. If this occurs, contact technical support immediately for assessment and potential replacement.
No, liquid nitrogen biological containers are specifically designed for LN2 storage. Using other liquids may damage the insulation, compromise the vacuum seal, and contaminate stored samples.
Only polycarbonate (PC) cryoboxes are recommended, as they are specifically designed to withstand ultra-low temperatures without cracking. Other materials may become brittle and fail under cryogenic conditions.
Various accessories are available to enhance usability and safety, including cryogenic gloves for hand protection, a five-wheel roller base for easy mobility, and a level gauge ruler for accurate LN2 level monitoring. These accessories help ensure safe and efficient handling of the container.