Polymerase Chain Reaction (PCR) cabinet provides HEPA-filtered vertical laminar flow to purge the work area of contaminants between amplifications and during preparatory procedures.
Esco offers premium performance for a price that is competitive with conventional "dead" air boxes. Its PCR cabinet's HEPA-filtered laminar flow and UV decontamination technology provides superior protection against cross-contamination within the main chamber making it the proven solution for contaminant-free PCR.
In practice, all polymerase chain reaction (PCR) procedures are conducted in the same room. Under these circumstances, PCR Cabinets are used for reagent preparation and sample preparation to minimize contamination. Because of the high copy number generated during PCR, it is essential to prevent possible contamination of the PCR.
Polymerase Chain Reaction is fundamental to almost all applications requiring a high copy number of starting material and is used in all laboratories working with DNA and RNA. Because of the high copy number generated during PCR, it is essential to prevent possible contamination of the PCR .
SCR-_A_
Streamline® Polymerase Chain Reaction Cabinet
Specifications:
- HEPA-filtered laminar flow
- ISO Class 3 work zone
- Equipped with German-made ebm-papst® motors with external rotor design
- UV decontamination technology
PCR-_A_
Airstream® Polymerase Chain Reaction Cabinet
Specifications:
- Pre-filter and main HEPA filter with a typical efficiency of >99.99% at 0.3 microns
- Protection against cross-contamination within the main chamber
- Built-in UV lamp with timer
- Sentinel™ Silver Microprocessor controller
- Available sizes: 3 ft, 4 ft
Dead-air boxes are non-ventilated enclosure with still or dead-air inside the workzone. Esco PCR cabinets provide HEPA-filtered vertical laminar airflow to the workzone.
PCR mastermix preparation, UV decontamination of PCR consumables
Streamline® and Airstream® Polymerase Chain Reaction Cabinets use H13 HEPA filter, with efficiency of 99.99% at between 0.1 to 0.3 microns.
Both vertical laminar flow bench and PCR cabinets serve the same purpose, that is to provide clean air environment and both are suitable to do the work. Vertical laminar bench provides more features such as:
· Electrical sockets in the workzone
· Provision for service fixtures
· Larger work area
· Brighter work surface
· Fan with larger reserve capacity
No. PCR cabinets do not provide user protection and cannot be used with infectious materials. These are mainly used for PCR mastermix and reagent preparation.
Yes. Although is not advisable to customize the Polymerase Chain Reaction Cabinets to have electrical outlets mainly because the electrical panel of the unit does not have any provision for this.
No. PCR cabinets do not provide user protection and cannot be used with infectious materials. These are mainly used for PCR mastermix and reagent preparation.
PCR model: The timer can be set from 0-18 hrs (timed only) with 1 minute increments.
SCR model: The timer can be set from 0-1 hr by using the UV timer knob.
The UV radiation intensity is at least 40 μW/cm2. At the centre of the cabinet, the radiation level is at least 1.2 mW/cm2.
Yes, PCR cabinet pre-filter is made of polyurethane which is washable.
The estimated shelf life of HEPA or ULPA filters is up to 10 years when stored properly.
Filters should be kept in their original packaging and should not be exposed to dampness, excessive heat, cold or rapidly changing temperatures. These filters can tolerate up to 95% humidity; anything higher can cause the filter media to block and fail.